Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori

摘要: Antibiotic resistance in bacteria incur fitness cost, but compensatory mechanisms may ameliorate the cost and sustain even under antibiotics-free conditions. The aim of this study was to determine antibiotic H. pylori. Five strains levofloxacin-sensitive pylori were induced vitro develop resistance. In addition, four pairs metronidazole-sensitive -resistant isolated from patients carrying dual populations that consist both sensitive resistant phenotypes. Growth rate, virulence biofilm formating ability compared effects response. Proteome profiles paired analyzed by liquid chromatography / mass spectrophotometry (LC/MS). Although there no significant differences growth rate between pairs, bacterial (in terms abilities induce apoptosis form biofilm) differs pair pair. These findings demonstrates complex strain-specific phenotypic changes compensation for antibiotics Compensation levofloxacin involving mutations gyrA gyrB functionally random. Furthermore, higher protein translation non-functional degradation capabilities naturally-occuring population metronidazole sensitive-resistant be a possible alternative mechanism underlying without rdxA frxA. This explain lack target genes approximately 10% strains.