Effects of antioxidants on Agrobacterium -mediated transformation and accelerated production of transgenic plants of Mexican lime ( Citrus aurantifolia Swingle)

摘要: Four antioxidants including glycine betaine, glutathione, lipoic acid, and polyvinylpyrrolidone were evaluated to improve transformation efficiency of Mexican lime, a precocious but recalcitrant citrus cultivar Agrobacterium mediated transformation. Lipoic acid substantially improved the lime by aiding in callus development improving shoot growth from cut ends epicotyl segments co-cultivated with Agrobacterium. Glycine betaine was moderately beneficial while glutathione did not have ability efficiency. A bi-functional gus-egfp fusion gene used study enabled visual identification transformants quantitative analysis expression. We describe an protocol that allows regenerated transgenic plants flower within 20–22 months after vitro regeneration. This rapid evaluation flowers fruits. Gene expression levels could be correlated copy number as determined using Southern blot analysis. Our method facilitates production plants, especially regarding functional transgenes citrus.