Cardiospecific microRNA plasma levels correlate with troponin and cardiac function in patients with ST elevation myocardial infarction, are selectively dependent on renal elimination, and can be detected in urine samples.

摘要: Objectives: Circulating microRNAs (miRNAs) are promising as biomarkers for various diseases. We examined the release patterns of cardiospecific miRNAs in a closed-chest, large animal ischemia-reperfusion model and patients with ST elevation myocardial infarction (STEMI). Methods: Six anesthetized pigs were subjected to coronary occlusion-reperfusion. Plasma, urine, clinical parameters collected from 25 STEMI undergoing primary percutaneous intervention. miRNA was extracted measured qPCR. Results: In pig reperfusion miR-1, miR-133a, miR-208b increased rapidly plasma peak at 120 min, while miR-499-5p remained elevated longer. all 4 abruptly 70-fold 3,000-fold plasma, within 12 h (p < 0.01). miR-1 miR-133a both correlated strongly glomerular filtration rate (GFR), indicating renal elimination. This confirmed by detection but not or miR-499-5p, urine. Peak values troponin ejection fraction. Conclusion: demonstrate distinct rapid increase levels circulation after infarction. Release cardiomyocyte necrosis markers, fraction, GFR, possible role these molecules diagnosis well prediction long-term complications.