Enhancement of membrane stability on magnetic responsive hydrogel microcapsules for potential on-demand cell separation.
作者： Huiyun Wen , Ting Gao , Zizhen Fu , Xing Liu , Jiatong xu
DOI: 10.1016/J.CARBPOL.2016.11.022
关键词: Rational design 、 Chitosan 、 On demand 、 Microsphere 、 Swelling 、 Chemical engineering 、 Cell separation 、 Membrane 、 Materials science 、 Bacillus licheniformis 、 Chromatography
摘要: Abstract It is of high interest to obtain hydrogel membranes with optimum mechanical stability, which a prerequisite the successful fabrication microcapsules for cell separation. In this work, we developed magnetic responsive alginate/chitosan (MAC) by co-encapsulation microbial cells and superparamagnetic iron oxide nanoparticles (SPIONs) reacting under voltage electrostatic field. We investigated influence molecular weight chitosan, size, membrane crosslinking time on swelling behavior as an indicator stability membranes. The results demonstrated that suitable conditions were obtained microspheres chitosan presenting 70 kDa 15–30 min resulting in thickness approximately 30 mm. Considering need maintaining inside microcapsules, fermentation at 37 °C neutral pH was favorable. Moreover, MAC sizing between 300 380 μm immobilizing Bacillus licheniformis 286 h multiple fed-bath operation no leakage SPIONs cells. Overall, study provided strategies rational design exhibiting stable
sci-hub.se 参考文章(35)
Weiting Yu, Huiyi Song, Guoshuang Zheng, Xiudong Liu, Ying Zhang, Xiaojun Ma, Study on membrane characteristics of alginate-chitosan microcapsule with cell growth Journal of Membrane Science. ,vol. 377, pp. 214- 220 ,(2011) , 10.1016/J.MEMSCI.2011.04.053
Jeane Chen, Sarah B. White, Kathleen R. Harris, Weiguo Li, Jonathan W.T. Yap, Dong-Hyun Kim, Robert J. Lewandowski, Lonnie D. Shea, Andrew C. Larson, Poly(lactide-co-glycolide) microspheres for MRI-monitored delivery of sorafenib in a rabbit VX2 model. Biomaterials. ,vol. 61, pp. 299- 306 ,(2015) , 10.1016/J.BIOMATERIALS.2015.05.010
Shaohua Zhang, Zhongyi Jiang, Xiaoli Wang, Chen Yang, Jiafu Shi, Facile Method To Prepare Microcapsules Inspired by Polyphenol Chemistry for Efficient Enzyme Immobilization. ACS Applied Materials & Interfaces. ,vol. 7, pp. 19570- 19578 ,(2015) , 10.1021/ACSAMI.5B03823
Thomas Leong, Linda Johansson, Pablo Juliano, Sally L. McArthur, Richard Manasseh, Ultrasonic Separation of Particulate Fluids in Small and Large Scale Systems: A Review Industrial & Engineering Chemistry Research. ,vol. 52, pp. 16555- 16576 ,(2013) , 10.1021/IE402295R
Barbara Krajewska, Membrane-based processes performed with use of chitin/chitosan materials Separation and Purification Technology. ,vol. 41, pp. 305- 312 ,(2005) , 10.1016/J.SEPPUR.2004.03.019
Jun Wu, Dequn Wu, Martha A. Mutschler, Chih-Chang Chu, Cationic Hybrid Hydrogels from Amino‐Acid‐Based Poly(ester amide): Fabrication, Characterization, and Biological Properties Advanced Functional Materials. ,vol. 22, pp. 3815- 3823 ,(2012) , 10.1002/ADFM.201103147
Yilong Wang, Fangyingkai Wang, Bingdi Chen, Hong Xu, Donglu Shi, Facile one-pot synthesis of yolk-shell superparamagnetic nanocomposites via ternary phase separations. Chemical Communications. ,vol. 47, pp. 10350- 10352 ,(2011) , 10.1039/C1CC13463A
Alexander D. Augst, Hyun Joon Kong, David J. Mooney, Alginate hydrogels as biomaterials. Macromolecular Bioscience. ,vol. 6, pp. 623- 633 ,(2006) , 10.1002/MABI.200600069
Sweta Rathore, Parind Mahendrakumar Desai, Celine Valeria Liew, Lai Wah Chan, Paul Wan Sia Heng, Microencapsulation of microbial cells Journal of Food Engineering. ,vol. 116, pp. 369- 381 ,(2013) , 10.1016/J.JFOODENG.2012.12.022
Jing Xiao, Ying Zhang, Jianzheng Wang, Weiting Yu, Wei Wang, Xiaojun Ma, Monitoring of cell viability and proliferation in hydrogel-encapsulated system by resazurin assay. Applied Biochemistry and Biotechnology. ,vol. 162, pp. 1996- 2007 ,(2010) , 10.1007/S12010-010-8975-3