Improved protocol for the simultaneous extraction and column-based separation of DNA and RNA from different soils
作者： Stefanie Töwe , Stefanie Wallisch , Andrea Bannert , Doreen Fischer , Brigitte Hai
DOI: 10.1016/J.MIMET.2010.12.028
关键词: RNA 、 Nucleic acid 、 Gene 、 Terminal restriction fragment length polymorphism 、 DNA 、 Restriction fragment length polymorphism 、 Biology 、 Molecular biology 、 Extraction (chemistry) 、 RNA extraction 、 Chromatography 、 Microbiology (medical) 、 Microbiology
摘要: … Briefly, 0.5 g of moist soil were extracted in Lysing Matrix E tubes (MP Biomedicals, France) with 0.5 ml of hexadecyltrimethylammoniabromide (CTAB) extraction buffer and 0.5 ml of …
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