Developmental capacity of mouse oocytes following maintenance of meiotic arrest in vitro

摘要: It was shown previously that the frequencies of fertilization and pre- post-implantation embryonic development mouse oocytes matured in vitro were similar to those vivo (Schroeder Eppig, Dev Biol 102:493–497, 1984). The present study determined developmental capacity after they had been maintained meiotic arrest by substances thought be important regulators meiosis vivo. Oocytes for 12 or 24 h medium containing maturation inhibitor(s), washed free inhibitor, cultured 16 inhibitor-free (control) permit maturation. Four different supplements used maintain arrest: (1) 100 μM dibutyryl cAMP plus 1 mM hypoxanthine; (2) 4 hypoxanthine 0.75 adenosine (H + AR); (3) 300 cAMP; (4) 50 IBMX. Parallel groups treated same experimental protocol except no inhibitory compounds used; eg, a total 28 40 control permitted resumption These latter tested effect extended culture mature on subsequent development. Control medium. inseminated subsequently assessed two-cell blastocyst stages. When first arrest, two-cells all but one within 10% controls (70%). H AR group exception (47% two-cells). By contrast, culturing resulted precipitous decrease two cells (27% 7%, respectively). Blastocyst followed pattern. uridine (U) added medium, increased significantly. Also, addition FSH significantly both U groups. Transfer compacted morulae from U/FSH into pseudopregnant hosts produced live young 19 days postinsemination. These data demonstrate prolonged decreased their undergo normal following insemination, if during then allowed spontaneously, potential preserved. results also lend support physiological role purines maintenance