Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
作者： A. F. Lima , G. May , J. Díaz-Colunga , S. Pedreiro , A. Paiva
DOI: 10.1038/S41598-018-25517-2
关键词: Cell biology 、 RNA polymerase II 、 Gene expression 、 Chemistry 、 Transdifferentiation 、 Cell fate determination 、 DNA 、 Transcription factor 、 Chromatin 、 Transcription (biology)
摘要: Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use osmotic modulation to modify chromatin reprogram In this study extracellular pressure as transcriptional modulator. Hyposmotic promotes loosening induces changes in RNA polymerase II (Pol II) activity. The decondensation opens space for higher amounts DNA engaged Pol II. constitutes an alternative route manipulate cell fate decisions. This technology was tested model protocols induced pluripotency transdifferentiation cells growing suspension adherent substrates, CD34+ umbilical-cord-blood (UCB), fibroblasts B-cells. efficiency kinetics these processes were improved by transient hyposmotic environment.
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