Hormone receptors and the control of cyclic AMP metabolism in parental and hybrid somatic cells.

摘要: Publisher Summary The study of cyclic AMP (cAMP) metabolism and its regulation by hormones in clonal cultured cell systems offers advantages over investigations more heterogeneous preparations. Cultured lines that display differentiated functions pertinent to the cAMP are readily available. conventional biochemical pharmacological experimentation is facilitated because these clones genetically homogenous. Furthermore, opportunity exists manipulate genetic composition such replicating lines. Thus, attempts being made several laboratories dissect system using hybrid somatic cells selection mutant or variant with specific alterations response pathway. This chapter reviews effects β -adrenergic agents prostaglandins on synthesis parental formed fusion appropriate strains. It defines relationship between receptors for adenylate cyclase. also analyzes responses catecholamines PGE 1 , receptor, guanine nucleotides binding receptors, activity, receptor lacking cyclase activity.