Studies on the interaction of heparin with lysozyme by multi-spectroscopic techniques and atomic force microscopy
作者： Lunfu Tian , Xiaoli Hu , Zhongfang Liu , Shaopu Liu
DOI: 10.1016/J.SAA.2015.09.026
关键词: Dynamic light scattering 、 Spectroscopy 、 Lysozyme 、 Circular dichroism 、 Lyso- 、 Chemistry 、 Fluorescence 、 Quenching (fluorescence) 、 Analytical chemistry 、 Absorbance
摘要: The interaction between heparin (Hep) and lysozyme (Lyso) in vitro was studied by fluorescence, UV-vis, circular dichroism (CD), resonance Rayleigh scattering (RRS) spectroscopy atomic force microscopy (AFM) under normal physiological conditions. UV-vis spectra of Lyso showed the absorbance significantly increased with addition Hep. Fluorescence studies revealed that emission quenching Hep initiated static mechanism. CD spectral induced conformational changes secondary structure Lyso. RRS intensity enhanced intensities were proportional to concentration a certain range. Thus, new method using as probe could be used for determination detection limit 3.9 ng mL(-1). In addition, shape complex characterized AFM. possible reaction mechanism reasons enhancement had been discussed through experimental results.
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