Occurrence of Clostridium spp. in ewe’s milk: enumeration and identification of isolates

摘要: The presence of gas-producing clostridia in ewe’s milk can lead to the occurrence late-blowing defects cheeses. However, data on this aspect are limited. In present study, using most probable number (MPN) method, clostridial spores were enumerated 527 samples collected Grosseto Province (Tuscany, Italy) from autumn 2014 summer 2015. addition, polymerase chain reaction (PCR), we identified species frequently involved cheese (Clostridium tyrobutyricum, Clostridium butyricum, beijerinckii, and sporogenes), perfringens. Gas-producing detected 99% samples. Spore concentrations ranged 360 more than 110,000 spores∙L−1. We observed that 86% had a spore load higher 1000 During summer, significantly winter spring (P < 0.001). A total 222 isolates obtained 77 MPN positive tubes different subjected PCR. Colonies 63/77 (82%) taxonomically identified. Among 63 PCR-positive samples, C. perfringens was (56%), followed by sporogenes (44%), tyrobutyricum (7.9%), butyricum (1.6%), beijerinkii (1.6%). 11% tubes, at least two found be simultaneously. This work highlights ovine central Italy (Tuscany) led identification some such high loads.