Detection of IgG antibody to Epstein-Barr virus viral capsid antigen in saliva by antibody capture radioimmunoassay
作者： A.J. Vyse , W.A. Knowles , B.J. Cohen , D.W.G. Brown
DOI: 10.1016/S0166-0934(96)02118-0
关键词: Epstein–Barr virus viral-capsid antigen 、 Virus 、 Radioimmunoassay 、 Saliva 、 Molecular biology 、 Antigen 、 Antibody 、 Monoclonal antibody 、 Biology 、 Epstein–Barr virus 、 Virology
摘要: A 'G' antibody capture radioimmunoassay (GACRIA) to detect IgG Epstein-Barr virus (EBV) viral capsid antigen (VCA) in saliva is described. The monoclonal EBV VCA used the GACRIA bound non-specifically when testing samples having a total content of less than 2.0 mg/l, so giving false positive results. This problem was overcome by including 0.5% EBV-negative human serum diluent. performance assay then evaluated comparing test on and indirect immunofluorescence (IFA) results sera using panel paired serum/saliva samples. Compared corresponding IFA had sensitivity specificity 93.5 100%, respectively. Although sensitive samples, sufficiently be for epidemiological screening will enable anti-EBV carried out easily wide scale.
elsevier.com
sci-hub.se 参考文章(14)
Richard F. Ambinder, Risa B. Mann, Detection and characterization of Epstein-Barr virus in clinical specimens American Journal of Pathology. ,vol. 145, pp. 239- 252 ,(1994)
R. F. Schinazi, W. R. Dowdle, A. J. Nahmias, The human herpesviruses: an interdisciplinary perspective. The human herpesviruses: an interdisciplinary perspective.. ,(1981)
Gertrude Henle, Werner Henle, Immunofluorescence in Cells Derived from Burkitt's Lymphoma Journal of Bacteriology. ,vol. 91, pp. 1248- 1256 ,(1966) , 10.1128/JB.91.3.1248-1256.1966
Principles and practice of clinical virology Principles and practice of clinical virology.. ,(1999) , 10.1002/9780470741405
Keith R. Perry, David W. G. Brown, John V. Parry, Sohrab Panday, Christopher Pipkin, Alison Richards, Detection of measles, mumps, and rubella antibodies in saliva using antibody capture radioimmunoassay Journal of Medical Virology. ,vol. 40, pp. 235- 240 ,(1993) , 10.1002/JMV.1890400312
P. P. Mortimer, J. V. Parry, Non‐invasive virological diagnosis: Are saliva and urine specimens adequate substitutes for blood? Reviews in Medical Virology. ,vol. 1, pp. 73- 78 ,(1991) , 10.1002/RMV.1980010204
Annika Andersson, Veronika Vetter, Ludger Kreutzer, Georg Bauer, Avidities of IgG directed against viral capsid antigen or early antigen: useful markers for significant Epstein-Barr virus serology Journal of Medical Virology. ,vol. 43, pp. 238- 244 ,(1994) , 10.1002/JMV.1890430308
R.A. Parker, D.D. Erdman, L.J. Anderson, Use of mixture models in determining laboratory criterion for identification of seropositive individuals: application to parvovirus B19 serology. Journal of Virological Methods. ,vol. 27, pp. 135- 144 ,(1990) , 10.1016/0166-0934(90)90130-8
N. Calvert, F. Cutts, R. Irving, D. Brown, J. Marsh, E. Miller, Measles immunity and response to revaccination among secondary school children in Cumbria Epidemiology and Infection. ,vol. 116, pp. 65- 70 ,(1996) , 10.1017/S0950268800058969
Q. Y. Yao, M. Rowe, A. J. Morgan, C. K. Sam, U. Prasad, H. Dang, Y. Zeng, A. B. Rickinson, Salivary and serum IgA antibodies to the Epstein-Barr virus glycoprotein gp340: incidence and potential for virus neutralization. International Journal of Cancer. ,vol. 48, pp. 45- 50 ,(2007) , 10.1002/IJC.2910480109