The Microscope, Spectra, and Automated Analysis

摘要: Fluorescence spectroscopy (FS) and optical rotatory dispersion (ORD) have been added to the capabilities of microscope. With them, as with conventional spectroscopy, a single component can be detected, even when in mixture. This ability is enhanced by use certain fluorescent dyes or "molecular probes" such acridine orange (AO). Molecular complexes between dye substrate may characteristic physical-optical physical-chemical properties. The microscope used for FS ORD studies produce fluorescence- ORD-spectra from selected portions unfixed cells tissues extremely small amorphous samples. Characteristic spectra obtained little 10-17 moles sample. It possible obtain cytochemical data that closely resembles, both spectroscopically numerically, solution on vitro model systems, consequently it predict quantitatively results interaction dye. Previous work cytochemistry cannot correlations, nor useful quantitative prediction. Data so corrected instrumental parameters, design automated analytical systems capable detecting quantitating particular biopolymers an intact cell. When biopolymer serve (qualitatively quantitatively) characterize cell type, identification, counting, separation readily devised predetermined fluorescence microscopic samples determine complexity systems. Not only relatively simple instruments sometimes serve, but automation analysis definitive avoided.