Serum albumin and its acid hydrolysis peptides dominate preparations of mineral-bound enamel proteins.

摘要: Serum albumin is a major noncollagenous protein component of bone, dentine, and, according to our results, enamel. Preparations mineral-bound proteins from porcine developing enamel contain single 67 kD at neutral pH or three (67, 63, and 53 kD) low that were assumed be enamelins, minor class proteins. A more complete analysis these in this study showed they derived serum (PSA). This was demonstrated by amino acid analysis, N-terminal sequence immunoblot studies using an anti-PSA antibody, SDS-PAGE the hydrolysis, cyanogen bromide, tryptic peptides. Examination different developmental stages PSA deposited organ dentine during development, not dissection process. These results indicate true enamelins must represent very small fraction total matrix teeth, has important implications on role mineralization