Production of monoclonal antibodies against enamelin and against amelogenin proteins of developing enamel matrix.

作者: D. Deutsch , A. Palmon , J. Catalano-Sherman , R. Laskov

DOI: 10.1177/08959374870010021801

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摘要: The extracellular matrix of developing enamel contains two major classes proteins, the hydrophobic proline-rich amelogenins and acidic serine-, glycine-, aspartic-rich enamelins. These proteins have been postulated as playing a role in mineralization structural organization enamel. To identify further characterize these different their possible this complex process biological mineralization, we recent years concerned with production specific probes for proteins. Previously, reported on successful polyclonal antibodies against enamelin which did not cross-react amelogenins, amelogenin enamelins (Deutsch et al., 1986, 1987). We now report monoclonal bovine protein (28 kDa) (66 kDa). One antibody am...

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