A High-Throughput Oxidative Stress Biosensor Based on Escherichia coli roGFP2 Cells Immobilized in a k-Carrageenan Matrix

摘要: Biosensors fabricated with whole-cell bacteria appear to be suitable for detecting bioavailability and toxicity effects of the chemical(s) concern, but they are usually reported have drawbacks like long response times (ranging from hours days), narrow dynamic range instability during term storage. Our aim is fabricate a sensitive oxidative stress biosensor which has improved properties that address mentioned weaknesses. In this paper, we report novel high-throughput by immobilizing roGFP2 expressing Escherichia coli cells in k-carrageenan matrix, detection challenged metalloid compounds. The E. shows high sensitivity towards arsenite selenite, wide linear low limit (arsenite: 1.0 × 10−3–1.0 101 mg·L−1, LOD: 2.0 10−4 mg·L−1; selenite: 10−5–1.0 102 5.8 10−6 mg·L−1), short (0–9 min), stability reproducibility. This research expected provide new direction performing environmental screening living bacterial capable measuring stressors friction second.