Effects of Fixative and Fixation Protocols on Assessment of Her-2/neu Oncogene Amplification Status by Fluorescence In Situ Hybridization
作者： Carlynn Willmore-Payne , Ken Metzger , Lester J. Layfield
DOI: 10.1097/01.PAI.0000209866.20581.8E
关键词: Molecular biology 、 Breast carcinoma 、 In situ hybridization 、 Her 2 neu oncogene 、 Fluorescence in situ hybridization 、 Newly diagnosed 、 Fixation (histology) 、 Biology 、 Fish analysis 、 Fixative
摘要: Fluorescence in situ hybridization (FISH) is used to determine amplification status of the Her-2/neu gene specimens newly diagnosed breast carcinoma. The Vysis kit for FISH analysis stipulates that tissue be formalin-fixed and paraffin-embedded. Concerns regarding carcinogenicity formal
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