Chitosan derivatives for gene transfer: effect of phosphorylcholine and diethylaminoethyl grafts on the in vitro transfection efficiency
作者： Isadora Pfeifer Dalla Picola , Qin Shi , Júlio Cesar Fernandes , Maicon Segalla Petrônio , Aline Margarete Furuyama Lima
DOI: 10.1080/09205063.2016.1225333
关键词: Nanoparticle 、 Organic chemistry 、 Nuclear chemistry 、 Chitosan 、 Materials science 、 Lipofectamine 、 In vitro transfection 、 DNA 、 Phosphorylcholine 、 Gene transfer
摘要: AbstractThe purpose of this work was to improve the functional properties chitosan for gene transfer by inserting phosphorylcholine (PC) and diethylaminoethyl (DEAE) groups into main chain. A series derivatives containing increasing contents DEAE a fixed content PC were synthesized characterized, aiming evaluate effect these on nanoparticles’ in vitro transfection efficiency. The soluble at physiological pH levels all less cytotoxic than control, lipid lipofectamine. obtained complexed pDNA nanoparticles with smaller sizes higher zeta potentials plain chitosan. performed prepared 6.3 7.4 results showed that 20% (PC18-CH) high degrees substitution (PC20-CH-DEAE100, CH-DEAE80, CH-DEAE100) displayed better transfecti...
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