A new type of hemophore-dependent heme acquisition system of Serratia marcescens reconstituted in Escherichia coli.
作者： J M Ghigo , S Létoffé , C Wandersman
DOI: 10.1128/JB.179.11.3572-3579.1997
关键词: Mutant 、 Secretion 、 Serratia marcescens 、 Porphyrin 、 Biology 、 Biochemistry 、 Heme 、 Bacterial outer membrane 、 Escherichia coli 、 Hemoglobin
摘要: The utilization by Serratia marcescens of heme bound to hemoglobin requires HasA, an extracellular heme-binding protein. This unique acquisition system was studied in Escherichia coli hemA mutant that a auxotroph. We identified 92-kDa iron-regulated S. outer membrane protein, HasR, which alone enabled the E. grow on or as porphyrin source. concomitant secretion HasA HasR-producing greatly facilitates from hemoglobin. is first report synergy between protein and acting carrier, we termed hemophore.
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sci-hub.se 参考文章(28)
Kathleen Postle, TonB protein and energy transduction between membranes. Journal of Bioenergetics and Biomembranes. ,vol. 25, pp. 591- 601 ,(1993) , 10.1007/BF00770246
I. Stojiljkovic, K. Hantke, Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria. The EMBO Journal. ,vol. 11, pp. 4359- 4367 ,(1992) , 10.1002/J.1460-2075.1992.TB05535.X
Carl A. Schnaitman, Solubilization of the Cytoplasmic Membrane of Escherichia coli by Triton X-100 Journal of Bacteriology. ,vol. 108, pp. 545- 552 ,(1971) , 10.1128/JB.108.1.545-552.1971
Jeffrey H. Miller, A short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria Published in <b>1992</b> in New York NY) by Cold Spring Harbor laboratory press. ,(1992)
D P Henderson, S M Payne, Characterization of the Vibrio cholerae outer membrane heme transport protein HutA: sequence of the gene, regulation of expression, and homology to the family of TonB-dependent proteins. Journal of Bacteriology. ,vol. 176, pp. 3269- 3277 ,(1994) , 10.1128/JB.176.11.3269-3277.1994
J.M. Ghigo, C. Wandersman, A carboxyl-terminal four-amino acid motif is required for secretion of the metalloprotease PrtG through the Erwinia chrysanthemi protease secretion pathway. Journal of Biological Chemistry. ,vol. 269, pp. 8979- 8985 ,(1994) , 10.1016/S0021-9258(17)37064-3
S Létoffé, J M Ghigo, C Wandersman, Secretion of the Serratia marcescens HasA protein by an ABC transporter. Journal of Bacteriology. ,vol. 176, pp. 5372- 5377 ,(1994) , 10.1128/JB.176.17.5372-5377.1994
Carl A. Schnaitman, Effect of Ethylenediaminetetraacetic Acid, Triton X-100, and Lysozyme on the Morphology and Chemical Composition of Isolated Cell Walls of Escherichia coli Journal of Bacteriology. ,vol. 108, pp. 553- 563 ,(1971) , 10.1128/JB.108.1.553-563.1971
I Stojiljkovic, J Larson, V Hwa, S Anic, M So, HmbR outer membrane receptors of pathogenic Neisseria spp.: iron-regulated, hemoglobin-binding proteins with a high level of primary structure conservation. Journal of Bacteriology. ,vol. 178, pp. 4670- 4678 ,(1996) , 10.1128/JB.178.15.4670-4678.1996
M. L. Smith, J. Paul, P. I. Ohlsson, K. Hjortsberg, K. G. Paul, Heme-protein fission under nondenaturing conditions Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 882- 886 ,(1991) , 10.1073/PNAS.88.3.882