Nitrate content and nitrate reductase activity in Rumex obtusifolius L. : I. Differences in organs and diurnal changes.
作者： G. Gebauer , A. Melzer , H. Rehder
DOI: 10.1007/BF00379795
关键词: Food science 、 Nitrate 、 Enzyme assay 、 Biology 、 Rumex 、 Nitrogen 、 Nitrate reductase 、 Botany 、 Rumex obtusifolius 、 Propanol 、 Incubation
摘要: With Rumex obtusifolius L., the influence of some environmental conditions on nitrate uptake and reduction were investigated. Nitrate concentrations plant material determined by HPLC, activity reductase an “in vivo” test. As optimal incubation medium, a buffer containing 0.04 M KNO3; 0.25 KH2PO4; 1.5% propanol (v/v); pH 8.0 was found. Vacuum infiltration caused increase enzyme up to 40%.
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