Fluorescence spectroscopy of turbid media: Autofluorescence of the human aorta.

摘要: Fluorescence spectra of turbid media depend on the geometry excitation and collection. The dependence 476-nm excited fluorescence human arterial wall was investigated both experimentally with a Monte Carlo simulation. Optical properties yield each three layers were determined. Attenuation by wavelength dependent scattering reabsorption causes observed at tissue surface to change distance from beam. ratio 600-nm 580-nm increases significantly beyond This depends amount oxyhemoglobin in sample, illustrating how can influence autofluorescence measurements. effects different excitation/collection geometries are discussed relation design catheters differentiate normal pathologic tissues.