Antibody class switching mediated by yeast endonuclease-generated DNA breaks.
作者： A. A. Zarrin , C. Del Vecchio , E. Tseng , M. Gleason , P. Zarin
关键词: Cytidine deaminase 、 Immunoglobulin heavy chain 、 DNA repair 、 Molecular biology 、 Endonuclease 、 Activation-induced (cytidine) deaminase 、 Locus (genetics) 、 Mutant 、 Immunoglobulin class switching 、 Biology
摘要: Antibody class switching in activated B cells uses switch recombination (CSR), which joins activation-induced cytidine deaminase (AID)–dependent double-strand breaks (DSBs) within two large immunoglobulin heavy chain (IgH) locus (S) regions that lie up to 200 kilobases apart. To test postulated roles of S and AID CSR, we generated mutant donor Sμ accepter Sγ1 were replaced with yeast I-SceI endonuclease sites. We found site-specific DSBs mediate recombinational IgH from IgM IgG1 without or AID. propose CSR evolved exploit a general DNA repair process promotes joining widely separated chromosome.
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