Expression of Common Gamma Chain on Peripheral Blood Mononuclear Cells in Crohn's Disease
作者： Irena Kirman , Ole Haagen Nielsen
关键词: Common gamma chain 、 Immunology 、 Interleukin 2 、 Cell activation 、 Receptor 、 Peripheral blood mononuclear cell 、 Flow cytometry 、 Monoclonal antibody 、 Medicine 、 Pathogenesis
摘要: Mononuclear cell proliferative response to IL-2and functional IL-4 is disturbed in IBD. Theaim of the present study was verify whether alteredexpression common gamma chain(γc) might contribute this abnormality. Theγc expression on peripheral bloodmononuclear cells analyzed by flow cytometry usinga monoclonal antibody γc. IL-4binding association with γcexpression assessed using biotinylated intwo-color cytometry. Mean fluorescence etaexpression significantly decreased active CDpatients as compared inactive CD and healthy controls(P < 0.05). Cell activation a possible reason forγc down-regulation confirmed usingthe vitro stimulated donor cells. In same systemit shown that γcis accompanied numbers binding Inconclusion, theγc blood mononuclearcells from patients disease have animportant pathogenetic significance chronicintestinal disorder.
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sci-hub.se 参考文章(29)
C P Braegger, T T MacDonald, Immune mechanisms in chronic inflammatory bowel disease. Annals of allergy. ,vol. 72, pp. 135- 141 ,(1994)
K. Itoh, X. Han, N. R. Pellis, Charles M Balch, Recombinant interleukin 4 (RIL4) inhibits interleukin 2-induced activation of peripheral blood lymphocytes. Lymphokine research. ,vol. 7, pp. 227- 235 ,(1988)
Shigeru Tsuchiya, Naoto Ishii, Masataka Nakamura, Yutaka Kimura, Toshikazu Takeshita, Michiyuki Maeda, Kazuo Sugamura, Takashi Uchiyama, Tasuke Konno, Hironobu Asao, Impairment of ligand binding and growth signaling of mutant IL-2 receptor gamma-chains in patients with X-linked severe combined immunodeficiency. Journal of Immunology. ,vol. 153, pp. 1310- 1317 ,(1994)
J.G. Giri, M. Ahdieh, J. Eisenman, K. Shanebeck, K. Grabstein, S. Kumaki, A. Namen, L.S. Park, D. Cosman, D. Anderson, Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15. The EMBO Journal. ,vol. 13, pp. 2822- 2830 ,(1994) , 10.1002/J.1460-2075.1994.TB06576.X
J S Economou, K Rhoades, D L Morton, R Essner, W H McBride, IL-4 down-regulates IL-1 and TNF gene expression in human monocytes. Journal of Immunology. ,vol. 142, pp. 3857- 3861 ,(1989)
Y Jacques, C Francois, F Boeffard, B Kaluza, U H Weidle, Construction of a bispecific antibody reacting with the alpha- and beta-chains of the human IL-2 receptor. High affinity cross-linking and high anti-proliferative efficiency. Journal of Immunology. ,vol. 150, pp. 4610- 4619 ,(1993)
Warren J. Leonard, Masayuki Noguchi, Sarah M. Russell, O. Wesley McBride, The Molecular Basis of X-Linked Severe Combined Immunodeficiency: The Role of the Interleukin-2 Receptor γ Chain as a Common γ Chain, γc Immunological Reviews. ,vol. 138, pp. 61- 86 ,(1994) , 10.1111/J.1600-065X.1994.TB00847.X
M Kondo, T Takeshita, N Ishii, M Nakamura, S Watanabe, K Arai, K Sugamura, Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. Science. ,vol. 262, pp. 1874- 1877 ,(1993) , 10.1126/SCIENCE.8266076
NIELS Tvede, JØRN Brynskov, Interleukin-2 stimulation of blood mononuclear cells from patients with inflammatory bowel disease. Apmis. ,vol. 99, pp. 759- 764 ,(1991) , 10.1111/J.1699-0463.1991.TB01256.X
Stefan Schreiber, Thomas Heinig, Ulf Panzer, Rüdiger Reinking, Anja Bouchard, Philipp D. Stahl, Andreas Raedler, Impaired response of activated mononuclear phagocytes to interleukin 4 in inflammatory bowel disease Gastroenterology. ,vol. 108, pp. 21- 33 ,(1995) , 10.1016/0016-5085(95)90004-7