Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer.

摘要: Events after fertilisation have been carefully studied in the last decades. However, there are still several questions to be clarified relation signalling pathway initiated by sperm, identification of proteins or factors involved activation arrested oocyte, and inactivation specific molecules meiotic arrest. The present state knowledge mammalian allows development protocols that closely mimic events sperm according certain major (MPF activity MAPk activity). These successfully used for oocytes NT giving rise offspring. Few cloned animals yet produced. pregnancy survival rates birth not significantly different when compared. This fact argues fora reason forthe low success efficiency NT. Eventually, related recipient donor cell culture conditions part these problems reconstructed embryo has overcome develop into a normal Nonetheless, imitate mechanism special interest improve developmental potential embryos.