A Molecular Surveillance Reveals the Prevalence of Vibrio cholerae O139 Isolates in China from 1993 to 2012

摘要: Vibrio cholerae serogroup O139 was first identified in 1992 India and Bangladesh, association with major epidemics of cholera both countries; cases were noted shortly thereafter China. We characterized 211 V. isolates that isolated at multiple sites China between 1993 2012 from patients (n = 92) the environment 119). Among clinical isolates, 88 (95.7%) 92 toxigenic, compared 47 (39.5%) 119 environmental isolates. Toxigenic carried El Tor CTX prophage toxin-coregulated pilus A gene (tcpA), as well seventh pandemic island I (VSP-I) VSP-II. a subset 42 toxigenic screened by multilocus sequence typing (MLST), all same type isolate (MO45) original Indian outbreak. Nontoxigenic contrast, generally lacked VSP-I -II, fell within13 additional types two clonal complexes distinct In further pulsed-field gel electrophoresis (PFGE) (with NotI digestion) studies, formed 60 pulsotypes clustered one group, while nontoxigenic 43 which into 3 different groups. Our data suggest widely divergent geographic locations, showing some diversity, have maintained relatively tight structure across 20-year time span. exhibited greater lineages, than did their counterparts.