Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans: identification of biomarkers and individual variability

摘要: Ellagitannins (ETs) are dietary polyphenols, containing ellagic acid (EA) subunits, with antioxidant and cancer chemopreventive activities that might contribute to health benefits in humans. However, little is known about their metabolic fate. We investigate here the metabolism of different ETs EA derivatives Forty healthy volunteers were distributed four groups. Each group consumed, a single dose, ET-containing foodstuff, i.e., strawberries (250 g), red raspberries (225 walnuts (35 oak-aged wine (300 mL). After intake, five urine fractions (F) collected at 8 (F1), 16 (F2), 32 (F3), 40 (F4), 56 (F5) h. Neither nor detected after LC-MS/MS analysis. microbial metabolite 3,8-dihydroxy-6H-dibenzo[b,d]pyran-6-one (urolithin B) conjugated glucuronic was along F3-F5 all subjects, independently consumed foodstuff. The mean percentage excretion ranged from 2.8 (strawberries) 16.6% (walnuts) regarding ingested ETs. Considerable interindividual differences noted, identifying "high low excreters" each group, which supported involvement colonic microflora ET metabolism. These results indicate urolithin B (a previously described antiangiogenic hyaluronidase inhibitor compound) biomarker human exposure may be useful intervention studies products. anticarcinogenic effects should considered gastrointestinal tract whereas study potential systemic focused on bioavailable derivatives.