Injectable non-immunogenic cartilage and bone preparation

摘要: Ground bone or cartilage particles are demineralized by extraction with a low ionic strength buffer such as 20 mM HEPES containing chelating agent and protease inhibitors, then extracted an acidic solution 0.3 M citric acid, pH 4.0, inhibitors. The material generally contains less than 2 % weight phosphate 100 calcium. content can be further reduced treatment of the matrix acid phosphatase, which removes residual organic phosphate. is useful in method vesicoureteral reflux other disorders where bulking effective correcting defect.