Methods and reagents for combined PCR amplification and hybridization probing

摘要: An oligonucleotide probe is disclosed, the including an oligonucleotide, a fluorescer molecule attached to first end of and quencher opposite oligonucleotide. The rendered impervious digestion by 5′→3′ exonuclease activity polymerase extension polymerase. invention also includes methods for performing combined PCR amplification hybridization probing, one such method steps contacting target nucleic acid sequence with reagents as described above, subjecting these thermal cycling. One preferred refinement above further addition strand displacer facilitate amplification. Additional similar are not requiring probes having their 5′ ends protected, wherein (i) lacks activity, (ii) inhibitor included, (iii) deactivation step performed.