Method for detection of target nucleic acids using pcr
作者： Martin Alan Lee , Dario Lyall Leslie
DOI:
关键词:
摘要: A method for detecting the presence of a target nucleic acid sequence in sample, said comprising (a) subjecting sample to an amplification reaction using set nucleotides, at least one which is fluorescently labelled, (b) contacting product with probe under conditions will hybridise sequence, reactive molecule able absorb fluorescence from or donate fluorescent energy labelled nucleotide and (c) monitoring sample. The can be used quantitate amount as well determine characteristics. Kits effecting are also claimed.
lens.org 参考文章(6)
Carl T. Wittwer, Philip S. Bernard, Multiplex genotyping using fluorescent hybridization probes ,(1999)
Chang-Ning J. Wang, Kai-Yuan Wu, Methods for reducing non-specific priming in DNA detection ,(1994)
Joseph L. Di Cesare, Fluorescence detection assay for homogeneous pcr hybridization systems ,(1997)
Paul E. Mayrand, Methods and reagents for combined PCR amplification and hybridization probing ,(1996)
Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen, Monitoring hybridization during pcr ,(1997)
Irina A. Nazarenko, Satish K. Bhatnagar, Robert J. Hohman, Emily S. Winn-Deen, Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon Official Gazette of the United States Patent and Trademark Office Patents. ,vol. 1219, pp. 482- ,(1997)