Transcriptional and post-transcriptional regulation of TcR, CD4 and CD8 gene expression during activation of normal human T lymphocytes.

摘要: We previously showed that the turnover rates of messengers coding for T cell receptor (TcR) alpha, beta and gamma, CD4 CD8 molecules composing multireceptor complex vary in normal human mature lymphocytes according to their state activation. Activation by soluble anti-CD3 which does not induce proliferation, promotes a weak up-modulation corresponding five mRNAs. In contrast, activation signals such as + PMA, lead lymphokine mRNA expression promote decrease TcR, levels within 4 h post-activation, followed gradual re-expression. Here we show down-modulation these mRNAs results from early regulation controls at transcriptional post-transcriptional levels, i.e. through concomitant inhibition transcription destabilization mRNA. Moreover, later re-expression recovery marked increase stability. Finally, is specific mRNAs, all submitted similar processes. These strongly suggest direct correlation between expression, cellular proliferation.