Lethal mutagenesis of HIV by mutagenic ribonucleoside analogs.

摘要: The extraordinarily high mutation rate of HIV suggests that the viral population exists near threshold for viability. An increase in could mediate a lethal already proportion defective viruses. We initially tested this hypothesis by culturing HIV-infected cells mutagenic deoxyribonucleoside analogs. In presence 5-hydroxydeoxycytidine, there was loss replication after sequential passages human CEM (Loeb LA, et al.: Proc Natl Acad Sci USA 1999;96:14921497). now propose to use ribonucleosides induce mutagenesis. incorporation analogs into genome host cell RNA polymerase, rather than reverse transcriptase, augurs well avoidance resistant