Identification of differentially expressed genes in the thoracic ganglion of the mud crab, Scylla paramamosain during ovarian maturation
作者： Hui Zeng , Jingru Huang , Wenxing Li , Huiyang Huang , Haihui Ye
DOI: 10.1080/17451000.2010.528775
关键词: Suppression subtractive hybridization 、 Biology 、 Scylla paramamosain 、 Bioinformatics 、 Gene 、 Thoracic ganglia 、 clone (Java method) 、 cDNA library 、 Complementary DNA 、 Molecular biology 、 Expressed sequence tag
摘要: Abstract The thoracic ganglion is an important neuroendocrine organ in crabs. In order to isolate and clone differentially expressed genes the of mud crab, Scylla paramamosain, during ovarian maturation, a forward suppression subtractive hybridization cDNA library was constructed (crabs at maturation stage were used as ‘tester’ those early developmental ‘driver’). obtained from inserted into expression vector transferred Escherichia coli. A total 500 randomly selected clones sequenced 410 sequence tags (ESTs) obtained. fragment size 200 1500 bp. insertion rate up 82%. This confirmed successful construction library. ESTs recognized based on BLAST searches NCBI, clustered assembled, annotated finally categorized. Among 139 genes, 124 (89.2%) similar known while 15 ...
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