B-type allatostatin regulates immune response of hemocytes in mud crab Scylla paramamosain

摘要: B-type allatostatins (AST-B) are neuropeptides that have important physiological roles in arthropods, they also been identified a number of crustacean species. Recent research on neuroendocrine-immune (NEI) regulatory system invertebrates has exploded, it reveals the NEI network plays an indispensable role optimizing immune response and maintaining homeostasis. Herein, mud crab Scylla paramamosain provides primitive ancient model to study crosstalk between neuroendocrine systems. In this study, qRT-PCR analysis showed nervous was main production site for Sp-AST-B mRNA S. paramamosain, while its receptor gene (Sp-AST-BR) could be detected all analyzed tissues including hemocytes. This AST-B might act as pleiotropic neuropeptide. situ hybridization further confirmed granular cells hemocyte subpopulations express Sp-AST-BR. Time-course revealed bacteria-analog LPS or virus-analog Poly (I:C) challenge significantly induced expression thoracic ganglion, Sp-AST-BR hemocytes were positively changed. Furthermore, crabs treated with synthetic peptide increased levels AST-BR, nuclear factor-κB (NF-κB) transcription factor (Dorsal Relish), pro-inflammatory cytokine (IL-16) immune-effector molecules, dramatically enhanced nitric oxide (NO) phagocytic activity Meanwhile dsRNA-mediated knockdown remarkably suppressed NO concentrations, related genes, resulting markedly impaired ability inhibit bacterial proliferation vivo. Combined, these data demonstrate innate crab.