Evaluation of fluorescence in situ hybridization for the detection of bacteria in feline inflammatory liver disease.

摘要: The etiopathogenesis of feline inflammatory liver disease (ILD) is unclear. Therefore, we sought to determine the presence and distribution bacteria within livers cats with ILD using eubacterial fluorescence in situ hybridization (FISH). Histopathology from 39 19 histologically normal (C) were classified World Small Animal Veterinary Association guidelines. Hepatic sections examined by 16 23S ribosomal RNA FISH. Antibodies against cytokeratins factor VIIIa used distinguish bile ducts vascular structures. Histopathologic findings included non-specific reactive hepatitis (12), neutrophilic cholangitis (NC; 12), lymphocytic (seven), cholestasis/obstruction (three), probable lymphoma (three) acute (two). Bacteria observed 21/39 3/19 C (P = 0.0054). In 8/39 2/19 restricted outer capsule 0.29) may represent contaminants. prevalence intrahepatic was higher 0.008) (13/31) than (1/17). more frequently <0.0001) localized portal vessels, venous sinusoids parenchyma (12/13) duct (1/13). Bacterial colonization highest Escherichia coli-positive NC cats. Concurrent non-hepatic disease, predominantly pancreatic intestinal (8/10 biopsied), present all 13 bacteria. culture positive (predominantly E coli Enterococcus species) 11/23 (48%) samples, concurred FISH 15/23 cases. 13/31 (41%) suggests a role etiopathogenesis. supports possibility via either enteric translocation or hematogenous seeding.