Genetic engineering of Enterobacter asburiae strain JDR-1 for efficient d (−) lactic acid production from hemicellulose hydrolysate
作者： C. Bi , X. Zhang , J. D. Rice , L. O. Ingram , J. F. Preston
DOI: 10.1007/S10529-009-0044-Z
关键词: Enterobacter 、 Xylose 、 Biochemistry 、 Hemicellulose 、 Fermentation 、 Food science 、 Biology 、 Mixed acid fermentation 、 Xylose metabolism 、 Xylan 、 Hydrolysate
摘要: In the dilute acid pretreatment of lignocellulose, xylose substituted with α-1,2-methylglucuronate is released as methylglucuronoxylose (MeGAX), which cannot be fermented by biocatalysts currently used to produce biofuels and chemicals. Enterobacter asburiae JDR-1, isolated from colonized wood, efficiently both MeGAX in hydrolysates sweetgum xylan. Deletion pflB als genes this bacterium modified native mixed fermentation pathways one for homolactate production. The resulting strain, L1, completely utilized a hydrolysate xylan produced lactate approximating 100% theoretical maximum yield. JDR-1 offers platform develop efficient production fuels chemicals hemicellulose hardwood agricultural residues.
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