Fine-Tunable and Injectable 3D Hydrogel for On-Demand Stem Cell Niche.

摘要: Stem-cell-based tissue engineering requires increased stem cell retention, viability, and control of differentiation. The use biocompatible scaffolds encapsulating cells typically addresses the first two problems. To achieve fate, fine-tuned with bioactive molecules are necessary. However, given that fine-tuning is associated UV irradiation in situ scaffold gelation, this process conflict injectability. Herein, a fine-tunable injectable 3D hydrogel system developed thermosensitive poly(organophosphazene) bearing β-cyclodextrin (β-CD PPZ) types adamantane-peptides (Ad-peptides) mesenchymal (MSC) differentiation serve as stoichiometrically controlled pendants for fine-tuning. Given complexation hosts guests subject to strict stoichiometric achieved simple mixing, these fabricated hydrogels exhibit well-aligned, responses, even living animals. Injection MSCs also results various chondrogenic levels at three weeks postinjection. This attributed differential controls Ad-peptides, if MSC preconditioning excluded. Eventually, could be applied platform technology by simply switching peptides adamantane their stoichiometry.