The H(+)-ATPase in the plasma membrane of Saccharomyces cerevisiae is activated during growth latency in octanoic acid-supplemented medium accompanying the decrease in intracellular pH and cell viability.

摘要: Saccharomyces cerevisiae plasma membrane H+-ATPase activity was stimulated during octanoic acid-induced latency, reaching maximal values at the early stages of exponential growth. The time-dependent pattern ATPase activation correlated with decrease cytosolic pH (pHi). cell population used as inoculum exhibited a significant heterogeneity pHi, and fall pHi loss viability determined by plate counts. When growth started, only fraction initial still viable, consistent role physiology number viable cells in duration latency under acid stress.