EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA "IN VITRO" DE UNA CEPA NATIVA DE Saccharomyces cerevisiae "IN VITRO" EVALUATION OF THE PROBIOTIC CAPACITY OF A NATIVE strain of Saccharomyces cerevisiae
作者： Joanna Reuto , Balkys Quevedo-Hidalgo , Sandra Sarmiento , Ángela Ortiz , Erika Fajardo
DOI:
关键词:
摘要: The in vitro probiotic capacity of a native strain Saccharomyces cerevisiae (A) was evaluated and compared with commercial (B) used as probiotic. effect the concentration sugarcane molasses (10, 20 30% (w/v)) on biomass production investigated kinetic parameters were determined. best 20% (w/v) differences medium between A (28 g/L) control B (3 observed. In tests such tolerance to bile salts, pH gastric juices carried out, no growth found. Cholesterol reduction presence salts after 12 hours incubation 54% for 58% B. Both strains showed adherence Caco-2 cells. Results reveal that possesses properties can be verified further vivo studies confirm its suitability animal nutrition.
参考文章(23)
Gerd Müller, Wolfgang Pannhorst, Ulrich Schiffner, The Scientific Basis Springer Berlin Heidelberg. pp. 13- 49 ,(1995) , 10.1007/978-3-662-03083-7_2
Cristina A. Viegas, Paulo F. Almeida, Miguel Cavaco, Isabel Sá-Correia, The H(+)-ATPase in the plasma membrane of Saccharomyces cerevisiae is activated during growth latency in octanoic acid-supplemented medium accompanying the decrease in intracellular pH and cell viability. Applied and Environmental Microbiology. ,vol. 64, pp. 779- 783 ,(1998) , 10.1128/AEM.64.2.779-783.1998
Alis van der Aa Kühle, Kerstin Skovgaard, Lene Jespersen, In vitro screening of probiotic properties of Saccharomyces cerevisiae var. boulardii and food-borne Saccharomyces cerevisiae strains. International Journal of Food Microbiology. ,vol. 101, pp. 29- 39 ,(2005) , 10.1016/J.IJFOODMICRO.2004.10.039
Keiji Mitsui, Hidetomo Yasui, Norihiro Nakamura, Hiroshi Kanazawa, Oligomerization of the Saccharomyces cerevisiae Na+/H+ antiporter Nha1p: Implications for its antiporter activity Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1720, pp. 125- 136 ,(2005) , 10.1016/J.BBAMEM.2005.11.005
Ana Rubio M, María Hernández E, Andréa Aguirre R, Raúl Poutou P, Identificación preliminar in vitro de propiedades probióticas en cepas de S. cerevisiae Revista Mvz Cordoba. ,vol. 13, pp. 1157- 1169 ,(2008) , 10.21897/RMVZ.406
Ryuichi Ohgaki, Norihiro Nakamura, Keiji Mitsui, Hiroshi Kanazawa, Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1712, pp. 185- 196 ,(2005) , 10.1016/J.BBAMEM.2005.03.011
Kozo Ouchi, Kazuhiko Takeshige, Factors affecting the ethanol productivity of yeast in molasses Journal of Fermentation and Bioengineering. ,vol. 79, pp. 449- 452 ,(1995) , 10.1016/0922-338X(95)91260-C
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
Mario Guslandi, Patrizia Giollo, Pier Alberto Testoni, A pilot trial of Saccharomyces boulardii in ulcerative colitis. European Journal of Gastroenterology & Hepatology. ,vol. 15, pp. 697- 698 ,(2003) , 10.1097/00042737-200306000-00017
Tomas Brandberg, Lena Gustafsson, Carl Johan Franzén, The impact of severe nitrogen limitation and microaerobic conditions on extended continuous cultivations of Saccharomyces cerevisiae with cell recirculation Enzyme and Microbial Technology. ,vol. 40, pp. 585- 593 ,(2007) , 10.1016/J.ENZMICTEC.2006.05.032