Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii
作者： Seunghye Park , Yew Lee , Jae-Hyeok Lee , EonSeon Jin
DOI: 10.1007/S00425-013-1955-4
关键词: Gene 、 Chlamydomonas reinhardtii 、 Sequence analysis 、 Biology 、 Molecular biology 、 Dunaliella 、 Transgene 、 Transcription (biology) 、 Chlamydomonas 、 Base pair
摘要: The development of highly inducible promoters is critical for designing effective transformation systems transgenic analyses. In this study, we investigated the promoter light-inducible protein gene (LIP) marine alga Dunaliella sp. LIPs are homologs early light-induced proteins (ELIPs) Arabidopsis thaliana. DNA sequence analysis revealed that LIP contains several light-responsive motifs. Constructs containing progressive truncations fused with a Renilla luciferase were introduced into Chlamydomonas reinhardtii to identify region in promoter. Transcription from was stimulated by high light (HL) intensity-dependent manner. contrast, oxidative stress induced chemicals had little effect on promoter, which implies exclusively light. Truncation −100 base pair (bp) abrogated inducibility, suggests presence negative cis-regulatory element upstream bp fragment. can be utilized research specifically select and propagate microalgae under high-light conditions.
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