High-Intensity-Light-Dependent and Transient Expression of New Genes Encoding Distant Relatives of Light-Harvesting Chlorophyll-a/b Proteins in Chlamydomonas reinhardtii

摘要: We identified four Lhc-like genes (Lhl) encoding proteins that are distant relatives of light-harvesting chlorophyll a/b-binding (LHC) in the green alga Chlamydomonas reinhardtii. Their mRNA levels after transfer from low-intensity light to high-intensity (HL) were examined and compared with those Lhc LHC PSII. The caused a decrease level Lhl3, homolog Arabidopsis thaliana Lil3, within 2 h, followed by gradual restoration depending on intensity HL. response was similar genes. In contrast, Lhl1, Lhl2 Lhl4 significantly increased, reached maximum 1 h then rapidly returned low level. HL little influenced these While Lhl1 homologs early light-inducible protein (ELIP) high-light-inducible (HLIP), respectively, encoded novel protein. HL-induced expression most prominent among tested. Studies using various inhibitors indicate is not mediated redox state plastoquinone pool or reactive oxygen species, but required de novo synthesis cytosol.