Application of reversibly soluble polymers in bioprocessing.

作者: M Fujii

DOI: 10.1016/0167-7799(91)90062-M

关键词: PolyelectrolyteReversible reactionOrganic chemistryPolymerEnzyme catalysisDownstream processingSolubilityAqueous solutionIonic strengthChemistry

摘要: Abstract Reversibly soluble polyelectrolytes, whose solubility in aqueous solutions is dependent on factors such as pH or ionic strength, may be used supports for enzymes affinity ligands. Their dual nature exploited to take advantage of the form during, examplw, enzymatic reactions, and insoluble during downstream processing operations enzyme product recovery.

参考文章(17)
Alexei L. MARGOLIN, Svetlana F. SHERSTYUK, Vladimir A. IZUMRUDOV, Alexander B. ZEZIN, Victor A. KABANOV, Enzymes in polyelectrolyte complexes. The effect of phase transition on thermal stability. FEBS Journal. ,vol. 146, pp. 625- 632 ,(1985) , 10.1111/J.1432-1033.1985.TB08697.X
M. Charles, R. W. Coughlin, F. X. Hasselberger, Soluble–insoluble enzyme catalysts Biotechnology and Bioengineering. ,vol. 16, pp. 1553- 1556 ,(1974) , 10.1002/BIT.260161113
Masayuki Taniguchi, Seiichi Tanahashi, Michihiro Fujii, Purification of staphylococcal protein a by affinity precipitation dissociation of protein a from the adsorbent with chemical reagents Journal of Fermentation and Bioengineering. ,vol. 69, pp. 362- 364 ,(1990) , 10.1016/0922-338X(90)90245-R
Masayuki Taniguchi, Mikio Kobayashi, Michihiro Fujii, Properties of a reversible soluble-insoluble cellulase and its application to repeated hydrolysis of crystalline cellulose. Biotechnology and Bioengineering. ,vol. 34, pp. 1092- 1097 ,(1989) , 10.1002/BIT.260340810
Robert W. Coughlin, Masuo Aizawa, Marvin Charles, Preparation and properties of soluble–insoluble nicotinamide coenzymes Biotechnology and Bioengineering. ,vol. 18, pp. 199- 208 ,(1976) , 10.1002/BIT.260180206
E. Van Leemputten, M. Horisberger, Soluble-insoluble complex of trypsin immobilized on acrolein-acrylic acid copolymer. Biotechnology and Bioengineering. ,vol. 18, pp. 587- 590 ,(1976) , 10.1002/BIT.260180410
Kazuhiro Hoshino, Masayuki Taniguchi, Yoshiaki Netsu, Michihiro Fujii, Repeated hydrolysis of raw starch using amylase immobilized on a reversibly soluble-insoluble carrier Journal of Chemical Engineering of Japan. ,vol. 22, pp. 54- 59 ,(1989) , 10.1252/JCEJ.22.54
C. Senstad, B. Mattiasson, Affinity-precipitation using chitosan as ligand carrier. Biotechnology and Bioengineering. ,vol. 33, pp. 216- 220 ,(1989) , 10.1002/BIT.260330212
Masayuki Taniguchi, Mikio Kobayashi, Katsumi Natsui, Michihiro Fujii, Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand Journal of Fermentation and Bioengineering. ,vol. 68, pp. 32- 36 ,(1989) , 10.1016/0922-338X(89)90210-9