作者: Robert W. Coughlin , Masuo Aizawa , Marvin Charles
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摘要: A soluble–insoluble form of nicotinamide adenine dinucleotide (NAD+), which can be rendered either soluble or insoluble by simply adjusting the pH, has been prepared covalently coupling NAD to alginic acid using 1,2,7,8-diepoxyoctane. The bound showed coenzymic function in state and could collected for further use as a precipitate lowering pH below 3. Coupling coenzymes with insolubilized apoenzymes is possible fluidized fixed-bed reactors.