Preparation and properties of soluble–insoluble nicotinamide coenzymes

作者: Robert W. Coughlin , Masuo Aizawa , Marvin Charles

DOI: 10.1002/BIT.260180206

关键词:

摘要: A soluble–insoluble form of nicotinamide adenine dinucleotide (NAD+), which can be rendered either soluble or insoluble by simply adjusting the pH, has been prepared covalently coupling NAD to alginic acid using 1,2,7,8-diepoxyoctane. The bound showed coenzymic function in state and could collected for further use as a precipitate lowering pH below 3. Coupling coenzymes with insolubilized apoenzymes is possible fluidized fixed-bed reactors.

参考文章(8)
M. Charles, R. W. Coughlin, F. X. Hasselberger, Soluble–insoluble enzyme catalysts Biotechnology and Bioengineering. ,vol. 16, pp. 1553- 1556 ,(1974) , 10.1002/BIT.260161113
J.M. Siegel, G.A. Montgomery, R.M. Bock, Ultraviolet absorption spectra of DPN and analogs of DPN. Archives of Biochemistry and Biophysics. ,vol. 82, pp. 288- 299 ,(1959) , 10.1016/0003-9861(59)90124-9
Borivoj. Janik, Philip J. Elving, Polarographic behavior of nucleosides and nucleotides of purines, pyrimidines, pyridines, and flavins. Chemical Reviews. ,vol. 68, pp. 295- 319 ,(1968) , 10.1021/CR60253A003
Per-Olof Larsson, Klaus Mosbach, Preparation of a NAD(H)-polymer matrix showing coenzymic function of the bound pyridine nucleotide Biotechnology and Bioengineering. ,vol. 13, pp. 393- 398 ,(1971) , 10.1002/BIT.260130306
Michael K. Weibel, Howard H. Weetall, Harold J. Bright, Insolubilized coenzymes: the covalent coupling of enzymatically active NAD to glass surfaces. Biochemical and Biophysical Research Communications. ,vol. 44, pp. 347- 352 ,(1971) , 10.1016/0006-291X(71)90606-1
Robert W. Coughlin, Masuo Aizawa, Bruce F. Alexander, Marvin Charles, Immobilized‐enzyme continuous‐flow reactor incorporating continuous electrochemical regeneration of NAD Biotechnology and Bioengineering. ,vol. 17, pp. 515- 526 ,(1975) , 10.1002/BIT.260170405
Masuo Aizawa, Robert W. Coughlin, Marvin Charles, Electrolytic regeneration of the reduced from the oxidized form of immobilized NAD Biotechnology and Bioengineering. ,vol. 18, pp. 209- 215 ,(1976) , 10.1002/BIT.260180207