Intracellular distribution of histone mRNAs in human fibroblasts studied by in situ hybridization.

摘要: Abstract We have used in situ hybridization to study the intracellular distribution of mRNAs for cell cycle-dependent core and H1 histone proteins human WI-38 fibroblasts. Because histones are abundant nuclear mRNA expression is tightly coupled DNA synthesis, it was interest determine whether localized near nucleus. Cells were hybridized with tritiated probes specific either H1, H4, actin, or poly(A)+ processed autoradiography. In exponentially growing cultures, fraction mRNA-positive cells correlated well S phase eliminated by hydroxyurea inhibition synthesis. Within individual label widely distributed throughout cytoplasm did not appear be more heavily concentrated However, appeared exhibit patchy, nonhomogeneous localization, a quantitative evaluation confirmed that grain distributions as uniform they after hybridizations mRNA. Actin also but differed from actin frequently most dense at outermost region narrow extensions. The localization less pronounced qualitatively very similar previously described chicken embryonic myoblasts We conclude facilitated protein synthesis nucleus there subtle discrete potentially functional differences histone, mRNAs.