Amine Oxidases of Microorganisms:Part VII. An Improved Purification Procedure and Further Properties of Amine Oxidase of Aspergillus niger

摘要: An improved purification procedure is described for preparation of crystalline amine oxidase in higher yields from the mycelial extract Aspergillus niger. The was accumulated mycelia fungus when it grown a medium containing n-butylamine as sole nitrogen source. fed alkali tank pH stat connected with jar fermenter, to maintain culture at 5.0~5.5 during incubation.The shown be inhibited by guanidine hydrochloride and dissociation enzyme occurred dialyzed against 6 m 0.1 mercaptoethanol. native its molecular weight 252,000 dissociated into three subunits each 85,000 dialysis.Pyridoxal derivatives were detected digest proteolysis followed acid hydrolysis. showed biological activity support growth Saccharomyces carlsbergensis. Fluore...