Diagnosis of benzimidazole resistance in Haemonchus contortus using allele-specific PCR technique

摘要: Molecular tools can be of great use in understanding the origin and nature underlying mechanisms benzimidazole resistance. In present study an allele-specific PCR was standardized to diagnose mutation (Phe Try) at residue 200 isotype 1 beta tubulin gene responsible for resistance Haemonchus contortus adult infective larvae. Once standardized, AS-PCR proved a powerful tool detect point mutations codon s-tubulin H. worms The technique effectively used genotyping susceptible resistant alleles from sheep.