Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns.
作者： Tomomasa Watanabe , Yukimasa Hayashi , Nobuaki Ogasawara , Takeshi Tomoita
DOI: 10.1007/BF00499116
关键词: Nucleotide 、 Mitochondrial DNA 、 EcoRV 、 Enzyme 、 Large white 、 Biology 、 Molecular biology 、 BglII 、 Restriction enzyme 、 Genetics 、 Cleavage (embryo)
摘要: Restriction endonuclease cleavage patterns of mitochondrial DNA (mtDNA) pigs and Japanese wild boars were analyzed using 17 enzymes which recognize six nucleotides. The map sites was made by double-digestion methods. Polymophism mtDNA detected in the digestion BglII, EcoRV, ScaI, StuI. restriction identical among breeds Landrace, Hampshire, Duroc I, Large White I (A type). II same as those (B A difference between type B found case three enzymes, StuI, nucleotide alterations them estimated more than six. On other hand, a from almost all EcoRV. We suggest that could result different origin boars, is, whether they European or Asian origin.
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