Research resource: Gene profiling of G protein-coupled receptors in the arcuate nucleus of the female.

摘要: The hypothalamic arcuate nucleus controls many critical homeostatic functions including energy homeostasis, reproduction, and motivated behavior. Although G protein-coupled receptors (GPCRs) are involved in the regulation of these functions, relatively few GPCRs have been identified specifically within nucleus. Here, using TaqMan low-density arrays we quantified mRNA expression nonolfactory mouse An unprecedented number (total 292) were found to be expressed, which 183 known 109 orphan GPCRs. GPCR genes expressed classified into several functional clusters hormone/neurotransmitter, growth factor, angiogenesis vasoactivity, inflammation immune system, lipid messenger receptors. plethora 5 structure-related classes class A (rhodopsin-like), B (adhesion), C (other GPCRs), nonsignaling 7-transmembrane chemokine-binding proteins, other proteins. Therefore, for first time, provide a quantitative estimate numerous Finally, as proof principle, documented function one receptor genes, glucagon-like peptide 1 (Glp1r), was highly Single-cell RT-PCR revealed that Glp1r localized proopiomelanocortin neurons, whole-cell recording 1-selective agonist exendin-4 robustly excited neurons. Thus, data emphasize complexity furthermore valuable resource future neuroendocrine/endocrine-related experiments.