Growth Inhibition of Human Tumor Cells in Soft-Agar Cultures by Treatment with Low Levels of Adenosine 5′-Triphosphate

摘要: Treatment of a variety human tumor cells in monolayer cultures with low levels (40 to 80 microM) adenosine 5'-diphosphate (ADP) or 5'-triphosphate (ATP) was recently shown produce arrest cellular growth the S phase cell cycle (E. Rapaport, J. Cell. Physiol., 114: 279-283, 1983). We now demonstrate that exposure two well-characterized colonic adenocarcinoma (HT-29 and SW-620) pancreatic (CAPAN-1 PANC-1) lines soft-agar exogenously supplied 5 20 microM ATP results substantial inhibition growth. Exposure ADP produces slightly smaller growth-inhibitory effects, while 5'-monophosphate have marginal effects on proliferation these systems. Successful demonstration requires use heat-inactivated fetal bovine serum, since normal serum possesses enzymatic activities which catalyze rapid degradation adenine nucleotides. Tumor assayed by well-established colony formation assay as well [3H]thymidine incorporation into acid-insoluble material. [3H]Thymidine is performed 4 14 days after plating correlates obtained assays. Due ectoenzymatic include adenosinetriphosphatase adenosinediphosphatase catalyzing dephosphorylation ADP, effective inhibit this system, widely claimed predict vivo response tumor, are lower than supplied. The previously characterized, well-differentiated HT-29) were exhibit higher chemosensitivity towards treatment did lesser-differentiated (PANC-1 SW-620).