Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line.
作者： Guoren Deng , Youyong Lu , Shiming Chen , Jing Miao , Guirong Lu
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摘要: The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma line, BGC-823. transforming gene cloned from the genomic library secondary transformants using in situ hybridization probe human Alu repeat sequence. This is homologous to protooncogene c-Ha-ras. activation lesion identified by sequence analysis as single nucleotide substitution thymine for guanine 12th codon. results valine glycine at amino acid Mr 21,000 protein.
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