H-ras and K-ras mutations in soft tissue sarcoma: Comparative studies of sarcomas from Korean and American patients

摘要: BACKGROUND The authors' recent investigation of Korean patients with sarcoma has suggested that ras gene activation may play a role in oncogenesis. The authors attempted to extend the mutation analysis sarcomas American determine whether there were racial or geographic factors relevant initiation progression sarcoma. METHODS H-ras and K-ras genes examined obtained from midwestern U. S. using polymerase chain reaction technique direct automated sequencing analysis. Tumors studied included 29 malignant fibrous histiocytomas, 7 liposarcomas, 5 rhabdomyosarcomas, 9 leiomyosarcomas. RESULTS Of 50 evaluated, only 1 (2%) definable was found; GGC AGC transition at codon 12 H-ras found rhabdomyosarcoma. None had mutation. rates incidence point mutations these samples much lower (H-ras: 2%; 95% confidence interval [95% CI], 0-11.5% K-ras: 0%) than described for both studies 16%; CI, 5.2-26.8% 44%; 29.5-58.5%). CONCLUSIONS Although reason this discrepancy is not clear, no major differences histology clinical stages. Based on study previous 45 tumor samples, conclude differing genetic and/or environmental mechanisms can affect development progression. Mutation appears be uncommon occurring patients, suggesting by does significant pathogenesis patients. Cancer 1999;86:58–63. © 1999 Society.