Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells
作者： Mitchell Goldfarb , Kenji Shimizu , Manuel Perucho , Michael Wigler
DOI: 10.1038/296404A0
关键词: Gene 、 Gene targeting 、 Restriction enzyme 、 Molecular biology 、 Cell culture 、 DNA 、 Gene expression 、 Blot 、 Regulation of gene expression 、 Biology
摘要: DNA from T24, a cell line derived human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells. Using techniques gene rescue to clone responsible for this transformation, we have found that it is in origin, less than 5 kilobase pairs size and homologous 1,100-base polyadenylated RNA species T24 HeLa Blot analysis indicates extensive restriction endonuclease polymorphism near gene, DNAs.
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T Tanaka, B Weisblum, Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. Journal of Bacteriology. ,vol. 121, pp. 354- 362 ,(1975) , 10.1128/JB.121.1.354-362.1975
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
B. Vogelstein, D. Gillespie, Preparative and analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 615- 619 ,(1979) , 10.1073/PNAS.76.2.615
Nat Sternberg, David Tiemeier, Lynn Enquist, In vitro packaging of a λ Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1 Gene. ,vol. 1, pp. 255- 280 ,(1977) , 10.1016/0378-1119(77)90049-X
Allan R. Goldberg, Martha Howe, New mutations in the S cistron of bacteriophage lambda affecting host cell lysis Virology. ,vol. 38, pp. 200- 202 ,(1969) , 10.1016/0042-6822(69)90148-2
H. O. Smith, M. L. Birnstiel, A simple method for DNA restriction site mapping Nucleic Acids Research. ,vol. 3, pp. 2387- 2398 ,(1976) , 10.1093/NAR/3.9.2387
Manuel Perucho, Douglas Hanahan, Michael Wigler, Genetic and Physical Linkage of Exogenous Sequences in Transformed-Cells Cell. ,vol. 22, pp. 309- 317 ,(1980) , 10.1016/0092-8674(80)90178-6
J. Bubeník, M. Barešová, V. Viklický, J. Jakoubková, H. Sainerová, J. Donner, Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. International Journal of Cancer. ,vol. 11, pp. 765- 773 ,(1973) , 10.1002/IJC.2910110327
A.D. Kaiser, David S. Hogness, The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdg Journal of Molecular Biology. ,vol. 2, pp. 392- IN6 ,(1960) , 10.1016/S0022-2836(60)80050-2
T. G. Krontiris, G. M. Cooper, Transforming activity of human tumor DNAs Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 1181- 1184 ,(1981) , 10.1073/PNAS.78.2.1181